11 episódios

Podcast of University of Minnesota Supercomputing Institute

University of Minnesota Supercomputing Institute University of Minnesota

    • Educação

Podcast of University of Minnesota Supercomputing Institute

    • video
    Configuring terminal client

    Configuring terminal client

    The following video provides instruction for connecting to MSI Linux machines using terminal clients.

    • 6 min
    • video
    NX client configuration

    NX client configuration

    The following video provides instruction for connecting to MSI Linux machines using NoMachine NX client.

    • 7 min
    • video
    MSI labs

    MSI labs

    The following video provides an overview of all the labs at MSI.

    • 3 min
    • video
    MSI Linux system configuration

    MSI Linux system configuration

    MSI is primarily a Linux based HPC center. The following video provides an overview of all the Linux systems at MSI.

    • 4 min
    • video
    Introduction to MSI

    Introduction to MSI

    This video is an introduction to MSI resources brought to you by MSI staff.

    • 20 min
    • video
    Module Five : False Discovery Rate Analysis

    Module Five : False Discovery Rate Analysis

    ProteinPilot™ is a proteomics software program used for analysis of peptide tandem mass spectrometry data and for relative quantification of proteins using the iTRAQ® technology and other modes of stable isotope labeling. Raw data acquired on AB Sciex instruments such as MALDI-TOF/TOF 4800 or QSTAR systems is searched against protein sequence databases; the Paragon scoring algorithm provides confidence levels for protein hits, and the ProGroup algorithm tool (PMID: 17533153) groups related and homologous proteins. The highlights of the software include: a user-friendly interface, simultaneous prediction of hundreds of peptide modifications (~175) and non-tryptic cleavages, the ability to perform quantification at the protein iso-form level, and a sophisticated FDR (false discovery rate) analysis that includes protein- and peptide-level reports. The newest version of ProteinPilot™ also supports identification of peptides and proteins from data acquired on non-AB Sciex instruments after conversion of raw data into *.mgf file format. The tutorial will include an introduction to the software followed by iTRAQ® Data analysis workflow and FDR analysis overviews.

    • 24 min

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