250 episodes

Die Universitätsbibliothek (UB) verfügt über ein umfangreiches Archiv an elektronischen Medien, das von Volltextsammlungen über Zeitungsarchive, Wörterbücher und Enzyklopädien bis hin zu ausführlichen Bibliographien und mehr als 1000 Datenbanken reicht. Auf iTunes U stellt die UB unter anderem eine Auswahl an elektronischen Publikationen der Wissenschaftlerinnen und Wissenschaftler an der LMU bereit. (Dies ist der 15. von 22 Teilen der Sammlung 'Medizin - Open Access LMU'.)

Medizin - Open Access LMU - Teil 15/22 Ludwig-Maximilians-Universität München

    • Education

Die Universitätsbibliothek (UB) verfügt über ein umfangreiches Archiv an elektronischen Medien, das von Volltextsammlungen über Zeitungsarchive, Wörterbücher und Enzyklopädien bis hin zu ausführlichen Bibliographien und mehr als 1000 Datenbanken reicht. Auf iTunes U stellt die UB unter anderem eine Auswahl an elektronischen Publikationen der Wissenschaftlerinnen und Wissenschaftler an der LMU bereit. (Dies ist der 15. von 22 Teilen der Sammlung 'Medizin - Open Access LMU'.)

    Untersuchungen zu Diagnostik und Therapie des Buruli Ulkus mittels tropenadaptierter Labormethoden in endemischen Regionen

    Untersuchungen zu Diagnostik und Therapie des Buruli Ulkus mittels tropenadaptierter Labormethoden in endemischen Regionen

    Intracapillary leucocyte accumulation as a novel antihaemorrhagic mechanism in acute pancreatitis in mice

    Intracapillary leucocyte accumulation as a novel antihaemorrhagic mechanism in acute pancreatitis in mice

    Background: Pancreatic infiltration by leucocytes represents a hallmark in acute pancreatitis. Although leucocytes play an active role in the pathophysiology of this disease, the relation between leucocyte activation, microvascular injury and haemorrhage has not been adequately addressed.Methods: We investigated intrapancreatic leucocyte migration, leucocyte extravasation and pancreatic microperfusion in different models of oedematous and necrotising acute pancreatitis in lys-EGFP-ki mice using fluorescent imaging and time-lapse intravital microscopy.Results: In contrast to the current paradigm of leucocyte recruitment, the initial event of leucocyte activation in acute pancreatitis was represented through a dose- and time-dependent occlusion of pancreatic capillaries by intraluminally migrating leucocytes. Intracapillary leucocyte accumulation (ILA) resulted in dense filling of almost all capillaries close to the area of inflammation and preceded transvenular leucocyte extravasation. ILA was also initiated by isolated exposure of the pancreas to interleukin 8 or fMLP, demonstrating the causal role of chemotactic stimuli in the induction of ILA. The onset of intracapillary leucocyte accumulation was strongly inhibited in LFA-1-/- and ICAM-1-/- mice, but not in Mac-1-/- mice. Moreover, prevention of intracapillary leucocyte accumulation led to the development of massive capillary haemorrhages and transformed mild pancreatitis into lethal haemorrhagic disease.Conclusions: ILA represents a novel protective and potentially lifesaving mechanism of haemostasis in acute pancreatitis. This process depends on expression of LFA-1 and ICAM-1 and precedes the classical steps of the leucocyte recruitment cascade.

    Interference microscopy delineates cellular proliferations on flat mounted internal limiting membrane specimens.

    Interference microscopy delineates cellular proliferations on flat mounted internal limiting membrane specimens.

    Aim: To demonstrate that interference microscopy of flat
    mounted internal limiting membrane specimens clearly
    delineates cellular proliferations at the vitreomacular
    interface.
    Methods: ILM specimens harvested during vitrectomy
    were fixed in glutaraldehyde 0.05% and paraformaldehyde
    2% for 24 h (pH 7.4). In addition to interference
    microscopy, immunocytochemistry using antibodies
    against glial fibrillar acidic protein (GFAP) and neurofilament
    (NF) was performed. After washing in phosphatebuffered
    saline 0.1 M, the specimens were flat-mounted
    on glass slides without sectioning, embedding or any
    other technique of conventional light microscopy. A cover
    slide and 49,6-diamidino-2-phenylindole (DAPI) medium
    were added to stain the cell nuclei.
    Results: Interference microscopy clearly delineates
    cellular proliferations at the ILM. DAPI stained the cell
    nuclei. Areas of cellular proliferation can be easily
    distinguished from ILM areas without cells.
    Immunocytochemistry can be performed without changing
    the protocols used in conventional microscopy.
    Conclusion: Interference microscopy of flat mounted ILM
    specimens gives new insights into the distribution of
    cellular proliferations at the vitreomacular interface and
    allows for determination of the cell density at the ILM.
    Given that the entire ILM peeled is seen en face, the
    techniques described offer a more reliable method to
    investigate the vitreoretinal interface in terms of cellular
    distribution compared with conventional microscopy.

    Comparison of CT colonography, colonoscopy, sigmoidoscopy and faecal occult blood tests for the detection of advanced adenoma in an average risk population.

    Comparison of CT colonography, colonoscopy, sigmoidoscopy and faecal occult blood tests for the detection of advanced adenoma in an average risk population.

    Definite multiple system atrophy in a German family

    Definite multiple system atrophy in a German family

    A variable neurodegenerative phenotype with polymerase gamma mutation

    A variable neurodegenerative phenotype with polymerase gamma mutation

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