Listen In - Bitesize Bio Webinar Audios Bitesize Bio

Volume electron microscopy or volume EM (vEM) was named one of Nature's Technologies to Watch in 2023. The new Thermo Scientific Hydra Bio PFIB integrates advanced cryo-TEM lamella preparation to volume EM workflows.
In this episode of Listen In, see how the new Thermo Scientific Hydra Bio Plasma-Focused Ion Beam (PFIB) extends cryo technology to volume EM and enables the acquisition of high-resolution 3D data through automated serial milling and imaging of frozen-hydrated specimens.
Cryo Auto Slice & View enables the acquisition of high-resolution 3D data through automated serial milling and imaging for striking new insights into a range of frozen-hydrated specimens prepared by high-pressure freezing or plunge freezing.
The choice of four different plasma ion species offers advantages for imaging cryo- and resin-embedded samples. The Hydra Bio also features the Spin Mill Bio Method, an exclusive technique for large-area planar milling that yields similar-sized imaging areas as microtome slicing. Plus, see how Hydra Bio PFIB combines field-proven and new techniques to enable cryo and room temperature applications.
Watch the full presentation here: https://events.bitesizebio.com/cryo-tomography-to-volume-em/room
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, see how emerging and global challenges of conducting ethical animal research have led to the 12Rs Framework to guide humane experimental techniques in animal studies and other research areas.
The Principles of Humane Experimental Technique, the 3Rs (Replacement, Refinement, and Reduction), have been the mainstay of humane research involving animals for over fifty years.
However, it is becoming increasingly apparent that to enable high-quality ethical and humane science involving animals, other ethical constructs and issues, for example, research culture, reproducibility, reliability, and responsibility, must also be considered.
The 12Rs Framework brings these different constructs together in a single visual framework. Furthermore, it applies to all areas of scientific research, not just research involving animals.
Watch the full presentation here: https://events.bitesizebio.com/the-12rs-framework-enabling/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, learn more about how the convergence of AI technology and scientific discovery can revolutionize our understanding of biology and disease.
Generating insights into tissue microenvironments is crucial to our understanding of normal and abnormal tissue development, such as during cancer progression.
Spatial biology methods using microscopy and multiplexing techniques have allowed researchers to bring together protein expression profiles and spatial information to dive into the complex biology needed for these insights. However, image analysis of these datasets, especially with high numbers of protein markers and/or large numbers of cells, remains a challenge.
Artificial Intelligence (AI) image analysis has significantly progressed through innovations in deep learning, segmentation, and detection, making it a powerful tool for researchers.
Hear about the latest release of Aivia image analysis software, including AI tools, to help you tackle this challenge in your large 2D multiplexed image datasets.
Watch the full presentation here: https://microscopyfocus.com/elevate-your-research-with-spatial-insights/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Glycosylation is a prevalent modification of proteins known to play diverse roles across a wide range of biological processes.
While glycoproteins are widely recognized as key molecular features involved in human health and disease, the analysis of protein glycosylation has historically been hampered by a lack of efficient and simple methods to characterize the analytically challenging glycan structures. Examples include structures exhibiting extensive heterogeneity and subtle isomeric features such as linkage and topological differences.
In this episode of Listen In, Dr. Thaysen-Andersen discusses opportunities and challenges in the burgeoning field of analytical glycoscience.
See the current landscape of glycobiology, learn about innovative analytical methods for N- and O-linked glycosylation profiling from biological specimens, and explore how advances in glycoanalytics enable new insights into human glycobiology.
MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.
Watch the full presentation here: https://events.bitesizebio.com/unleash-new-biological-insights/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Liquid chromatography-mass spectrometry (LC-MS) is a crucial technique in many life sciences, including proteomics, metabolomics, pharmaceutical analysis, clinical diagnosis, and forensic science.
In this episode of Listen In, elevate your analysis and liquid chromatography. Learn what consumables, materials, and reagents can harm your LC-MS data for more accurate quantification using perfluoroalkyl substances (PFAS) as an example.
See how contamination from consumables, equipment, reagents, and non-specific binding of samples to syringe filters can make LC-MS quantification inaccurate. Plus, explore the environmental impact of PFAS and how to choose syringe filters and other consumables for accurate quantification results.
Watch the full presentation here: https://events.bitesizebio.com/contamination-and-recovery-of-pfas/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Holotomography has emerged as a helpful tool for imaging live specimens without additional pre-treatment, such as fixation, fluorescence labeling, and excitation.
It can achieve long-term three-dimensional observations of live specimens for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved through synthetic numerical aperture provides sufficient spatial information to distinguish various subcellular compartments such as nuclei, nucleoli, mitochondria, and lipid droplets.
Furthermore, analysis of the measured individual cell data can elucidate the temporal 3D volumetric dynamics with the dry mass information.
This episode presents the latest development of a low-coherence holotomography imaging system, HT-X1, and its numerous applications to different types of biological specimens, ranging from unicellular organisms to multicellular specimens.
Plus, learn how to combine holotomography with downstream molecular analysis, such as cell biology, immunology, microbiology, material science, and in vitro diagnosis.
Watch the full presentation here: https://events.bitesizebio.com/label-free-3d-live-cell-imaging-and/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/