Wirkung von Enterococcus faecium auf den Organismus neonataler Hundewelpen Tierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 01/07
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The present study aimed to investigate the effect of oral administration of a probiotic containing Ec. faecium (Total concentration 1 x 108 cfu / 100 g dry matter of feed) on the development and health status of hand reared beagle puppies. For this experiment each control group, respectively each trial group consisted of 6 puppies. The trial began on day 3 after the birth of the puppies in order to ensure sufficient intake of colostrum, and ended on day 98. General health status, clinical parameters, daily feed intake, weight gain, amount of faeces and faeces consistency were recorded. Apart from that 8 blood samples were taken to determine immune status, and 4 for establishing blood parameters. Faeces samples were taken continually in order to determine the dry matter. At 4 different periods there were microbiological analyses as well as a determination of lactate and ammonia in each individual puppy’s faeces. To determinate a specific immune response, humane serumalbumin (hsa) was applied in week 8 and 12, and the serum concentration of total IgG, IgA and specific antibodies against hsa were measured.
The probiotic affected the puppies´ general health status slightly negatively during the first 8 days of the trail, resulting in sleepiness, yawning, reduced suckling, reduced muscular tone and reduced feed intake. Besides that no negative effects of the probiotic on the puppies´ health were discovered.
The feeding of Ec. faecium did neither influence weight gain nor feed intake. The trail group showed a 6.5 % better feed conversion during the period of mixed feeding with Pedigree® and milk-replacer.
No effect of the probiotic on red blood cells and thrombocytes was discovered, but the trial group showed on average a tendentious higher count on lymphocytes, neutrophil granulocytes and monocytes. Ec. faecium feeding resulted possibly in a raised number of macrophages, and herewith monocytes, a stronger immune response with more lymphocytes and a higher phagocytotic activity of neutrophil granulocytes. The lower IgG serum concentration of the trail group from week 2 on could be related to the probiotic, higher average values of IgA in week 2 and 4 suggested a stimulation of mucosal immunity by the additive.
In the trial group, the probiotic feeding seemed to result in a higher count of total aerobe bacteria with a lower share of E. coli, in higher Enterococcus counts and throughout lower shares of Cl. perfringens positive animals. These facts are as desirable for the maintenance of the intestinal eubiosis, as is the discovery of a higher average count on Lactobacillus spp. in the trail group. The count on aerobe bacteria, Cl. perfringens, Enterococcus spp. and Lactobacillus spp. decreased with ongoing age of the puppies. In the period of milk-replacer feeding, probiotic feeding seemed to result in a decrease of fecal dry matter.
Considering the high spread of single values, no significant effect of Ec. faecium on fecal lactate and ammonia concentration was discovered, although the probiotic seemed to lower fecal ammonia concentration at the beginning of the trail.
The present study aimed to investigate the effect of oral administration of a probiotic containing Ec. faecium (Total concentration 1 x 108 cfu / 100 g dry matter of feed) on the development and health status of hand reared beagle puppies. For this experiment each control group, respectively each trial group consisted of 6 puppies. The trial began on day 3 after the birth of the puppies in order to ensure sufficient intake of colostrum, and ended on day 98. General health status, clinical parameters, daily feed intake, weight gain, amount of faeces and faeces consistency were recorded. Apart from that 8 blood samples were taken to determine immune status, and 4 for establishing blood parameters. Faeces samples were taken continually in order to determine the dry matter. At 4 different periods there were microbiological analyses as well as a determination of lactate and ammonia in each individual puppy’s faeces. To determinate a specific immune response, humane serumalbumin (hsa) was applied in week 8 and 12, and the serum concentration of total IgG, IgA and specific antibodies against hsa were measured.
The probiotic affected the puppies´ general health status slightly negatively during the first 8 days of the trail, resulting in sleepiness, yawning, reduced suckling, reduced muscular tone and reduced feed intake. Besides that no negative effects of the probiotic on the puppies´ health were discovered.
The feeding of Ec. faecium did neither influence weight gain nor feed intake. The trail group showed a 6.5 % better feed conversion during the period of mixed feeding with Pedigree® and milk-replacer.
No effect of the probiotic on red blood cells and thrombocytes was discovered, but the trial group showed on average a tendentious higher count on lymphocytes, neutrophil granulocytes and monocytes. Ec. faecium feeding resulted possibly in a raised number of macrophages, and herewith monocytes, a stronger immune response with more lymphocytes and a higher phagocytotic activity of neutrophil granulocytes. The lower IgG serum concentration of the trail group from week 2 on could be related to the probiotic, higher average values of IgA in week 2 and 4 suggested a stimulation of mucosal immunity by the additive.
In the trial group, the probiotic feeding seemed to result in a higher count of total aerobe bacteria with a lower share of E. coli, in higher Enterococcus counts and throughout lower shares of Cl. perfringens positive animals. These facts are as desirable for the maintenance of the intestinal eubiosis, as is the discovery of a higher average count on Lactobacillus spp. in the trail group. The count on aerobe bacteria, Cl. perfringens, Enterococcus spp. and Lactobacillus spp. decreased with ongoing age of the puppies. In the period of milk-replacer feeding, probiotic feeding seemed to result in a decrease of fecal dry matter.
Considering the high spread of single values, no significant effect of Ec. faecium on fecal lactate and ammonia concentration was discovered, although the probiotic seemed to lower fecal ammonia concentration at the beginning of the trail.
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