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Glycosylation is a prevalent modification of proteins known to play diverse roles across a wide range of biological processes.
While glycoproteins are widely recognized as key molecular features involved in human health and disease, the analysis of protein glycosylation has historically been hampered by a lack of efficient and simple methods to characterize the analytically challenging glycan structures. Examples include structures exhibiting extensive heterogeneity and subtle isomeric features such as linkage and topological differences.
In this episode of Listen In, Dr. Thaysen-Andersen discusses opportunities and challenges in the burgeoning field of analytical glycoscience.
See the current landscape of glycobiology, learn about innovative analytical methods for N- and O-linked glycosylation profiling from biological specimens, and explore how advances in glycoanalytics enable new insights into human glycobiology.
MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.
Watch the full presentation here: https://events.bitesizebio.com/unleash-new-biological-insights/join
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Liquid chromatography-mass spectrometry (LC-MS) is a crucial technique in many life sciences, including proteomics, metabolomics, pharmaceutical analysis, clinical diagnosis, and forensic science.
In this episode of Listen In, elevate your analysis and liquid chromatography. Learn what consumables, materials, and reagents can harm your LC-MS data for more accurate quantification using perfluoroalkyl substances (PFAS) as an example.
See how contamination from consumables, equipment, reagents, and non-specific binding of samples to syringe filters can make LC-MS quantification inaccurate. Plus, explore the environmental impact of PFAS and how to choose syringe filters and other consumables for accurate quantification results.
Watch the full presentation here: https://events.bitesizebio.com/contamination-and-recovery-of-pfas/join
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Holotomography has emerged as a helpful tool for imaging live specimens without additional pre-treatment, such as fixation, fluorescence labeling, and excitation.
It can achieve long-term three-dimensional observations of live specimens for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved through synthetic numerical aperture provides sufficient spatial information to distinguish various subcellular compartments such as nuclei, nucleoli, mitochondria, and lipid droplets.
Furthermore, analysis of the measured individual cell data can elucidate the temporal 3D volumetric dynamics with the dry mass information.
This episode presents the latest development of a low-coherence holotomography imaging system, HT-X1, and its numerous applications to different types of biological specimens, ranging from unicellular organisms to multicellular specimens.
Plus, learn how to combine holotomography with downstream molecular analysis, such as cell biology, immunology, microbiology, material science, and in vitro diagnosis.
Watch the full presentation here: https://events.bitesizebio.com/label-free-3d-live-cell-imaging-and/join
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Buffers are critical to nearly all our experiments. Poorly prepared buffers lead to failed Western blots, poor protein yields, flat binding curves—and wasted time.
Understanding why you need buffers, knowing how to choose the right one, and troubleshooting buffer issues are fundamental skills you can apply to make all your experiments work better—and you only need to learn how to prepare them correctly once.
In this episode, dive deep into the world of chemical buffers. Explore how to select the best buffer for your experiments, share practical tips on preparing buffer solutions, and troubleshoot any hiccups that might come your way. Plus, we'll demystify key concepts like pH and pKa.
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You have written a research paper. What now? Your research can only have an impact if someone reads it. So, you must submit your article to a journal for publication.
In this episode of Listen In, learn the nuts and bolts of scientific publication with a step-by-step tour of the whole process. Explore how to select the right journal and what tools are available to help make that decision. Plus, get advice on how to make the journal submission process go smoothly and how to take critical reviews and turn them into positives in your re-submission.
Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-iv-1/join
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This is part four of our How To Write a Research Paper series:
I: Telling Your StoryII: The AnatomyIII: Common Problems And How To Overcome ThemIV: Understanding The Publication Process

The best way to solve a problem is to avoid it in the first place. While journals can and do reject articles for scientific reasons, they also reject articles for structural issues. By taking a few simple steps, you can learn how to circumvent these difficulties for a smooth publication process.
In this episode of Listen In, we'll go through some of the structural reasons that scientific journals reject articles, how to solve common structural issues, and additional steps you can take to help your journal reviewers.
Watch the full presentation here: https://events.bitesizebio.com/how-to-write-a-research-paper-iii-1/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
This is part three of our How To Write a Research Paper series:
I: Telling Your StoryII: The AnatomyIII: Common Problems And How To Overcome ThemIV: Understanding The Publication Process