Untersuchungen zum „Carry-over“ von Perfluorierten Tensiden aus Futtermitteln und Tränkwasser in tierische Lebensmittel am Modell der Legewachtel Tierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 05/07
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Female japanese quails (Coturnix coturnix japonica) were dosed for a period of six weeks with an equal amount of perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) through feed (190, 330, 580 or 1000 µg PFT/kg bw/d) or for a period of five weeks through drinking water (330 or 580 µg PFT/kg bw/d). Concentrations of PFOS and PFOA in eggs have been determined on day 0, 14 and 35 and those in liver and muscle tissue subsequently to the experimental period. Both compounds were accumulating in eggs and tissue probes in a dose related manner. In comparison to PFOA, PFOS was more effectively eliminated through the egg. Concentrations of PFOA measured on day 35 in eggs from the feed trial were 1597 ± 213, 3438 ± 234, 4880 ± 1210 and 11963 ± 3119 µg/kg dw respectively for dose groups 190 to 1000 and concentrations of PFOS were 3315 ± 54, 6591 ± 1016, 8983 ± 2133 and 29762 ± 5988 µg/kg dw respectively. Concentrations of PFOA measured in eggs of dose group 330 and 580 from the water trial were 6428 ± 452 and 11469 ± 595 µg/kg dw respectively and concentrations of PFOS were 11050 ± 3620 and 23643 ± 3071 µg/kg dw respectively. The PFOS:PFOA ratio has been nearly constant at a value of 2:1 through all dosing groups on day 14 and 35. In contrast, PFOA was more effectively accumulating in liver and muscle tissue than PFOS. The PFOA:PFOS ratio in muscle and liver tissue ranged from 1.5:1 to 2.3:1 and 0.9:1 to 1.8:1 respectively. The proportion of the total cumulative dose ranged from 1.7 to 5% for the concentration of PFOA in eggs on day 14 and from 0.8 to 3% on day 35. For PFOS the proportion of the total cumulative dose ranged from 3.5 to 14% on day 14 and from 1.5 to 4% on day 35 respectively. In the liver 0.4 to 0.8% of the total dose were found for PFOA and 0.2 to 0.8 for PFOS. The muscle tissue contained 0.4 to 1.6% of the total cumulative dose of PFOS. In contrast to eggs and liver tissue there has been a remarkable difference in accumulation of PFOA in muscle tissue concerning the uptake route. It ranged from 0.6 to 1.6% for admission through feed and from 3.2 to 3.6% for admission through drinking water.
Toxic effects were remarkable in dose group 1000 only and were displayed as weight loss, reduced feed consumption and reduced egg production. All toxic effects were completely reversible after cessation of dosing.
Female japanese quails (Coturnix coturnix japonica) were dosed for a period of six weeks with an equal amount of perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) through feed (190, 330, 580 or 1000 µg PFT/kg bw/d) or for a period of five weeks through drinking water (330 or 580 µg PFT/kg bw/d). Concentrations of PFOS and PFOA in eggs have been determined on day 0, 14 and 35 and those in liver and muscle tissue subsequently to the experimental period. Both compounds were accumulating in eggs and tissue probes in a dose related manner. In comparison to PFOA, PFOS was more effectively eliminated through the egg. Concentrations of PFOA measured on day 35 in eggs from the feed trial were 1597 ± 213, 3438 ± 234, 4880 ± 1210 and 11963 ± 3119 µg/kg dw respectively for dose groups 190 to 1000 and concentrations of PFOS were 3315 ± 54, 6591 ± 1016, 8983 ± 2133 and 29762 ± 5988 µg/kg dw respectively. Concentrations of PFOA measured in eggs of dose group 330 and 580 from the water trial were 6428 ± 452 and 11469 ± 595 µg/kg dw respectively and concentrations of PFOS were 11050 ± 3620 and 23643 ± 3071 µg/kg dw respectively. The PFOS:PFOA ratio has been nearly constant at a value of 2:1 through all dosing groups on day 14 and 35. In contrast, PFOA was more effectively accumulating in liver and muscle tissue than PFOS. The PFOA:PFOS ratio in muscle and liver tissue ranged from 1.5:1 to 2.3:1 and 0.9:1 to 1.8:1 respectively. The proportion of the total cumulative dose ranged from 1.7 to 5% for the concentration of PFOA in eggs on day 14 and from 0.8 to 3% on day 35. For PFOS the proportion of the total cumulative dose ranged from 3.5 to 14% on day 14 and from 1.5 to 4% on day 35 respectively. In the liver 0.4 to 0.8% of the total dose were found for PFOA and 0.2 to 0.8 for PFOS. The muscle tissue contained 0.4 to 1.6% of the total cumulative dose of PFOS. In contrast to eggs and liver tissue there has been a remarkable difference in accumulation of PFOA in muscle tissue concerning the uptake route. It ranged from 0.6 to 1.6% for admission through feed and from 3.2 to 3.6% for admission through drinking water.
Toxic effects were remarkable in dose group 1000 only and were displayed as weight loss, reduced feed consumption and reduced egg production. All toxic effects were completely reversible after cessation of dosing.
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