Biomanufacturing & Fermentation Technology

prasad ernala

Welcome to Biomanufacturing & Fermentation Technology, the podcast where microbes meet manufacturing and science turns into scalable reality. In each episode, we dive inside real bioprocesses. from lab-scale experiments to commercial fermenters. to unpack how products are actually made, fixed, and optimized in the real world. Expect candid conversations on fermentation failures and breakthroughs, scale-up war stories, regulatory realities, emerging technologies, and the decisions that separate a promising culture from a profitable process. Whether you are a scientist, engineer, entrepreneur, o

  1. BIOMANUFACTURING AND FERMENTATION TECHNOLOGY, The Bioprocess Pulse – (13–19 Feb 2026).

    2 DGN GELEDEN

    BIOMANUFACTURING AND FERMENTATION TECHNOLOGY, The Bioprocess Pulse – (13–19 Feb 2026).

    This week discussion summarizes a bioprocessing industry report highlighting significant technical and economic shiftsoccurring in early 2026. Researchers have successfully engineered yeast to overcome production barriers for industrial chemicals, while artificial intelligence is now being used to optimize genetic coding and streamline complex regulatory compliance. The report forecasts substantial market growth in hardware and infrastructure, driven by international investments and new government manufacturing initiatives in China. However, thesource also offers a critical reality check regarding the economic limitations of precision fermentation for low-value goods. Industry founders and engineers are encouraged to prioritize scalable technology and rigorous cost-benefit analyses to ensure long-term viability. Ultimately, these updates illustrate a transition toward data-driven biology and intensified manufacturing processes within the global bioeconomy. #Bioprocess #ScaleUp and #TechTransfer,#Industrial #Microbiology,#MetabolicEngineering and #SystemsBiology,#Bioprocessing,#MicrobialFermentation,#Bio-manufacturing,#Industrial #Biotechnology,#Fermentation Engineering,#ProcessDevelopment,#Microbiology,#Biochemistry,#Biochemical Engineering, #Applied #MicrobialPhysiology, #Microbial #ProcessEngineering, #Upstream #BioprocessDevelopment, #Downstream Processing and #Purification,#CellCulture and #MicrobialSystems Engineering, #Bioreaction #Enzymes, #Biocatalyst #scientific #Scientist #Research

    21 min
  2. Mechanistic Modeling and Mitigation of Fouling in Fermentation TFF

    3 DGN GELEDEN

    Mechanistic Modeling and Mitigation of Fouling in Fermentation TFF

    Hermia’s models categorize TFF fouling into four mechanisms, guiding regime identification and critical flux determination. Effective CIP protocols use caustic/acid washes to recover permeability. Digital twins combine mechanistic cores with AI to predict flux decline. How to use the result to minimize fouling in production TFF • Set your operating flux below the measured critical flux (often with a safety factor) to avoid sustained resistance growth. • If you observe a TMP “jump” pattern above certain fluxes, treat that as entering a new fouling regime and back off; TMP-jump behavior above critical/threshold flux is discussed as a characteristic signature in constant‑flux fouling studies. • Re-measure critical flux when any major variable changes (broth solids/viscosity, temperature, membrane lot, crossflow/channel geometry), because critical flux is not a membrane constant; it is an operating‑condition-dependent boundary. #Bioprocess #ScaleUp and #TechTransfer,#Industrial #Microbiology,#MetabolicEngineering and #SystemsBiology,#Bioprocessing,#MicrobialFermentation,#Bio-manufacturing,#Industrial #Biotechnology,#Fermentation Engineering,#ProcessDevelopment,#Microbiology,#Biochemistry,#Biochemical Engineering, #Applied #MicrobialPhysiology, #Microbial #ProcessEngineering, #Upstream #BioprocessDevelopment, #Downstream Processing and #Purification,#CellCulture and #MicrobialSystems Engineering, #Bioreaction #Enzymes, #Biocatalyst #scientific #Scientist #Research

    15 min
  3. Programming the Cell Factory: Aligning Cellular Decision-Making and Control

    4 DGN GELEDEN

    Programming the Cell Factory: Aligning Cellular Decision-Making and Control

    This episode outlines a shift in bioprocessing strategy from maintaining static setpoints to designing dynamic environmental trajectories that align with internal cellular decision-making. Rather than treating microbes as passive catalysts, the author argues that scientists should use benchtop bioreactors to program specific patterns of stress, growth rates, and nutrient feeds. By treating variables like dissolved oxygen dips and temperature shifts as intentional design tools, researchers can better predict how populations will behave at an industrial scale. This discussion focuses on managing phenotypic subpopulations and metabolic burdens to ensure cells prioritize product synthesis over repair. Ultimately, the source provides a framework for using timed perturbations and phased growth strategies to achieve higher yields and more reproducible fermentation outcomes. #Bioprocess #ScaleUp and #TechTransfer,#Industrial #Microbiology,#MetabolicEngineering and #SystemsBiology,#Bioprocessing,#MicrobialFermentation,#Bio-manufacturing,#Industrial #Biotechnology,#Fermentation Engineering,#ProcessDevelopment,#Microbiology,#Biochemistry,#Biochemical Engineering, #Applied #MicrobialPhysiology, #Microbial #ProcessEngineering, #Upstream #BioprocessDevelopment, #Downstream Processing and #Purification,#CellCulture and #MicrobialSystems Engineering, #Bioreaction #Enzymes, #Biocatalyst #scientific #Scientist #Research

    22 min
  4. TFF Failure Mechanisms and Digital Twin Diagnostics

    5 DGN GELEDEN

    TFF Failure Mechanisms and Digital Twin Diagnostics

    This episode examines the technical challenges and failure mechanisms associated with using tangential flow filtration (TFF) for processing fermentation broths. It details how microfiltration (MF), ultrafiltration (UF), and diafiltration (DF) are impacted by dynamic fouling, ranging from initial pore blocking to irreversible cake compaction. The discussion emphasize that scaling these processes to an industrial level often introduces hydrodynamic inconsistencies and logistical complexities that are not present in laboratory settings. To address these issues, the text proposes the use of digital twins and AI frameworks to monitor hidden resistance states and identify regime shifts in real time. Ultimately, these advanced models allow for targeted mitigation strategies that improve membrane longevity and process efficiency during large-scale production. #Bioprocess #ScaleUp and #TechTransfer,#Industrial #Microbiology,#MetabolicEngineering and #SystemsBiology,#Bioprocessing,#MicrobialFermentation,#Bio-manufacturing,#Industrial #Biotechnology,#Fermentation Engineering,#ProcessDevelopment,#Microbiology,#Biochemistry,#Biochemical Engineering, #Applied #MicrobialPhysiology, #Microbial #ProcessEngineering, #Upstream #BioprocessDevelopment, #Downstream Processing and #Purification,#CellCulture and #MicrobialSystems Engineering, #Bioreaction #Enzymes, #Biocatalyst #scientific #Scientist #Research

    19 min
  5. Tangential Flow Filtration: Industrial Principles and Fermentation Applications

    6 DGN GELEDEN

    Tangential Flow Filtration: Industrial Principles and Fermentation Applications

    This episode serves as a comprehensive guide to Tangential Flow Filtration (TFF) within the context of industrial fermentation, moving from foundational mechanics to advanced digital applications. It defines TFF as a pressure-driven separation process where fluid flows parallel to the membrane to mitigate, though not entirely prevent, the accumulation of debris and fouling. The discussion details the critical interplay between operating parameters like transmembrane pressure and crossflow velocity, emphasizing that improper balance can lead to irreversible membrane damage or cell lysis. Beyond basic hardware, the sources explore specific applications in microfiltration, ultrafiltration, and diafiltration for product clarification and concentration. Finally, the text highlights the shift toward digital-twin technology and AI to predict fouling regimes and optimize processing stability in complex biological environments. #Bioprocess #ScaleUp and #TechTransfer,#Industrial #Microbiology,#MetabolicEngineering and #SystemsBiology,#Bioprocessing,#MicrobialFermentation,#Bio-manufacturing,#Industrial #Biotechnology,#Fermentation Engineering,#ProcessDevelopment,#Microbiology,#Biochemistry,#Biochemical Engineering, #Applied #MicrobialPhysiology, #Microbial #ProcessEngineering, #Upstream #BioprocessDevelopment, #Downstream Processing and #Purification,#CellCulture and #MicrobialSystems Engineering, #Bioreaction #Enzymes, #Biocatalyst #scientific #Scientist #Research

    20 min
  6. Precision Predators: Phage Therapy for Industrial Bioreactor Control

    15 FEB

    Precision Predators: Phage Therapy for Industrial Bioreactor Control

    Industrial bioreactors face significant economic and operational risks due to microbial contamination, which traditional broad-spectrum sterilization methods often fail to eliminate entirely. This discussion explores the emerging strategy of industrial phage therapy, which utilizes specific viral predators to selectively target and destroy unwanted bacteria without harming the production organisms. By transitioning from general exclusion to precision-engineered control, manufacturers can potentially improve process robustness and product quality while reducing their reliance on antibiotics. The research detail the mechanisms of phage action, the genetic and engineering challenges of scaling these solutions, and the necessary regulatory frameworks for implementation. Ultimately, the adoption of phages represents a shift toward more sustainable and resilient biomanufacturing through the active management of microbial ecosystems. This evolution in hygiene relies on integrating high-throughput screening and advanced monitoring to defend complex industrial environments. #Bioprocess #ScaleUp and #TechTransfer,#Industrial #Microbiology,#MetabolicEngineering and #SystemsBiology,#Bioprocessing,#MicrobialFermentation,#Bio-manufacturing,#Industrial #Biotechnology,#Fermentation Engineering,#ProcessDevelopment,#Microbiology,#Biochemistry,#Biochemical Engineering, #Applied #MicrobialPhysiology, #Microbial #ProcessEngineering, #Upstream #BioprocessDevelopment, #Downstream Processing and #Purification,#CellCulture and #MicrobialSystems Engineering, #Bioreaction #Enzymes, #Biocatalyst #scientific #Scientist #Research

    18 min
  7. Industrial Fermentation Sterile Boundary Management and Contamination Dynamics

    14 FEB

    Industrial Fermentation Sterile Boundary Management and Contamination Dynamics

    In this episode we explores sterile boundary management as a continuous, evolving challenge in industrial fermentation rather than a one-time achievement. My analysis highlights that boundary crossings, such as sampling and feeding, act as repeated stress tests that can lead to subclinical contamination, where foreign microbes subtly distort a culture’s metabolism without triggering traditional alarms. These sources argue that sterility is a dynamic system property influenced by mechanical wear, automation failures, and the cumulative risks inherent in long production campaigns. To combat these hidden threats, the text suggests monitoring diagnostic signatures like respiratory trends and substrate decoupling to detect leaks early. Ultimately, maintaining a sterile environment requires a transition from simple procedural compliance to a reliability engineering approach that accounts for equipment fatigue and operational frequency. #Bioprocess#ScaleUp and #TechTransfer, #Industrial#Microbiology, #MetabolicEngineeringand #SystemsBiology, #Bioprocessing, #MicrobialFermentation, #Bio-manufacturing, #Industrial#Biotechnology, #FermentationEngineering, #ProcessDevelopment, #Microbiology,#Biochemistry #BiochemicalEngineering, #Applied#MicrobialPhysiology, #Microbial#ProcessEngineering, #Upstream#BioprocessDevelopment, #DownstreamProcessing and #Purification, #CellCultureand #MicrobialSystems Engineering, #Bioreaction#Enzymes #Biocatalyst #scientific #Scientist #Research

    17 min
  8. Bio-manufacturing and Fermentation Technology. (2026 February 2nd week edition)

    13 FEB

    Bio-manufacturing and Fermentation Technology. (2026 February 2nd week edition)

    This intelligence brief from 2nd week February 2026 identifies actionable breakthroughs in bioprocessing and biocatalysis that prioritize practical scalability over speculative science. The report highlights significant process optimizations, such as high-density microbial fermentation strategies for GLP-1 analogues and advanced metabolic engineering to achieve high titers of toxic intermediates. Key technical shifts include moving toward continuous manufacturing and using spatial oxygen mapping to resolve long-standing issues with large-scale tank heterogeneity. Beyond the lab, the text examines the macroeconomic landscape, noting major pharmaceutical acquisitions and leadership changes that signal a push toward accessible cell therapies. Finally, it outlines a regulatory shift where digital data integrity and continuous processing are becoming the new industry standards for approval. Combined, these updates provide a roadmap for reducing costs and navigating the "valley of death" between pilot and commercial production. #Bioprocess #ScaleUp and #TechTransfer,#Industrial #Microbiology,#MetabolicEngineering and #SystemsBiology,#Bioprocessing,#MicrobialFermentation,#Bio-manufacturing,#Industrial #Biotechnology,#Fermentation Engineering,#ProcessDevelopment,#Microbiology,#Biochemistry,#Biochemical Engineering, #Applied #MicrobialPhysiology, #Microbial #ProcessEngineering, #Upstream #BioprocessDevelopment, #Downstream Processing and #Purification,#CellCulture and #MicrobialSystems Engineering, #Bioreaction #Enzymes, #Biocatalyst #scientific #Scientist #Research

    16 min
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Info

Welcome to Biomanufacturing & Fermentation Technology, the podcast where microbes meet manufacturing and science turns into scalable reality. In each episode, we dive inside real bioprocesses. from lab-scale experiments to commercial fermenters. to unpack how products are actually made, fixed, and optimized in the real world. Expect candid conversations on fermentation failures and breakthroughs, scale-up war stories, regulatory realities, emerging technologies, and the decisions that separate a promising culture from a profitable process. Whether you are a scientist, engineer, entrepreneur, o

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