UBO, PKB, FKB, mite washes, Harbo assays... It's an alphabet soup out there of ways to test your bees for hygienic traits and resistance to varroa. You may be testing to find out when to apply mite treatments or a brood break to a colony and in that case a simple mite wash is usually enough. But you may be testing to find out your very best genetics you have to raise next years queens for your yards and in that case, you probably need need more information than a mite wash provides. Or do you? Do these assays work, how reliably do they work and which one is the most reliable, if any? Ang Roell and Bi Kline of They Keep Bees are working to find out with a new NE SARE grant comparison study. Today Ang and I talk about the cornucopia of testing methods that each have their complications and limitations. We'll also find out what Ang and Bi will explore in this ongoing study. Plus, we chat bees, of course. :-) Hope you enjoy this weeks episode! kind regards, Leigh The Study (read the full grant proposal and study design here) In 2025 national honey bee losses exceeded 60% in commercial beekeeping operations. Miticide resistance was discovered in 100% of tested samples, highlighting an urgent need for biology-based strategies that reduce chemical dependence while strengthening colony resilience. This project evaluates the reliability and heritability of four major hygienic behavior assays, in order to determine which methods best predict mite resistance and overwinter survival. We will compare assay performance in twelve breeder colonies (six selected, six controls) and their untreated F1 daughters. Across two seasons, all colonies will undergo standardized rounds of UBO, PKB, and FKB testing, as well as mite washes and Harbo assays. By analyzing correlations among assay scores, mite loads, reproductive mite presence, and overwinter survival, we will identify which tools reliably capture heritable Varroa-resistant traits and which factors influence assay consistency. This project addresses a documented need for effective selection tools that help beekeepers identify and propagate Varroa-resistant stock. Results will be shared through a report, digital outreach, and a presentation at Cornell's Dyce Lab. A hands-on workshop at They Keep Bees will train beekeepers to practice each assay method. By providing evidence-based guidance to at least 100 beekeepers, the project aims to enable measurable improvements such as higher overwinter survival and reduced reliance on miticides. Project objectives from proposal: Primary Research Questions Do pin-killed brood (PKB), freeze-killed brood using liquid nitrogen (FKB), and unhealthy brood odor (UBO) assays provide predictive measures of heritable mite-resistant traits in F1 daughter queens? Are these assays also predictive of overall colony mite loads and winter survival outcomes? Winter survival will be defined as survival from August of year 1 until March of year 2. Thank you to the patrons who keep this podcast available to all (and they also get extra goodies as a thank you for the support). Not a patron yet? Please join us at https://www.patreon.com/fiveapple
