Absolute Gene-ius

Jordan Ruggieri, Lisa Crawford

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Inspiring stories and advice from dPCR gene-iuses.

15 juil.

qPCR, nested PCR, and the search for bacterial serotypes

Some science stories start with a textbook. Donna Paznar’s started with a tick. In this episode of Absolute Gene-ius, Donna Paznar, head of Molecular Infection Diagnostics at the Medical University of Vienna, discusses her work studying Streptococcus pneumoniae and its impact across the Austrian population. She explains why this respiratory bacterium can be especially dangerous for infants and older adults, how children may act as asymptomatic reservoirs, and why tracking more than 100 bacterial serotypes is both important and technically challenging. Donna also walks through the molecular methods behind her work, including qPCR for identifying positive samples, nested PCR for targeting more specific DNA regions, and sequencing to help confirm serotypes. Along the way, she highlights the complexity of surveillance research and the goal of developing more efficient multiplex qPCR approaches. The career corner takes a fun turn into ticks, workplace cliques, qPCR mistakes, and a proud sleuthing moment involving a rare tick-borne pathogen. Donna’s advice is simple and useful: try opportunities, expect failure, and stay open-minded. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
1 juil.

How PCR, CRISPR, and animal models are advancing oncology research

Cancer research is moving fast, and molecular tools are helping scientists see more, measure more, and ask better questions. In this Science Snapshot episode of Absolute Gene-ius, Jordan Ruggieri and Lisa Crawford revisit memorable oncology-related clips from past conversations. Dr. Raquel Munoz explains how CAR-T cells are engineered to recognize and kill cancer cells, and how PCR-based methods can help confirm successful modification and track CAR-T expansion. Dr. Dustin Rubinstein shares how CRISPR and genome editing are being used to create pig models, including “onco-pig,” that may better reflect aspects of human cancer biology than traditional models. The episode also connects cell-free DNA research from Dr. Lee Ann Baxter-Lowe to oncology applications, before turning to Dr. Kathie Sollweck’s discussion of circulating tumor DNA and the sensitivity needed to detect low mutational burden in liquid biopsy research. Finally, Valeria Rangel offers practical perspective on multiplex PCR assay design. And because it is Absolute Gene-ius, the science comes with some laughs, a few pig jokes, and a reminder that even complex oncology methods can be surprisingly fun to talk about. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
17 juin

Genotoxicity, vectors, and the power of integration site analysis

In this episode, Michael Stump and Matteo Franco from ProtaGene explore the growing importance of integration site analysis (ISA) in cell and gene therapy. As viral vectors like lentivirus and AAV become more widely used, understanding where and how they integrate into the genome is essential for assessing genotoxicity and long-term safety. The discussion dives into how sequencing reveals integration locations, clonal expansion risks, and potential activation of oncogenes. They also highlight how tools like digital PCR complement sequencing as orthogonal methods that help enable precise quantification, validation, and new applications like vector integrity and copy number analysis. Beyond the science, the conversation explores the CRO role in supporting both early-phase discovery and late-stage regulatory needs, balancing innovation with regulatory expectations, and adapting to emerging modalities like LNPs. The career corner brings a mix of honesty and humor, from chaotic CRO workdays to broken PCR capillaries and runaway lab mice, paired with thoughtful advice on navigating industry paths, building GMP expertise, and finding the right fit between academia and industry. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
8 juin

From brewing to bioprocessing – Steve Lewis on molecular biology trends

PCR may be decades old, but it is still one of the most versatile tools in modern molecular biology. In this crossover episode, Absolute Gene-ius welcomes Steve Lewis, host of Speaking of Mol Bio and Director of Product Management at Thermo Fisher Scientific. Steve discusses why PCR remains so broadly relevant across research and applied science, from human identification and food testing to gene synthesis and molecular characterization. He also explores emerging trends shaping molecular biology workflows, including multiplexing, assay design, customization, miniaturization, and the ongoing need for high-quality PCR plastics. The conversation also digs into sustainability in the lab, including why single-use plastics remain essential for reducing contamination risk and how more sustainable material sourcing can help reduce carbon impact without compromising performance. In Career Corner, Steve shares a winding path that started in communications, moved through startups and home brewing, and eventually led to bioprocessing, biosecurity, synthetic biology, and product management. His advice? Cultivate curiosity, surround yourself with good people, and try not to drop your E. coli flask. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
20 mai

Epigenetics unlocked – how behavior shapes biology

Dr. Robert Philibert joins the show to unpack the fascinating world of epigenetics, where environmental factors like smoking, alcohol, and lifestyle reshape how genes are expressed. He explains how methylation, histone modifications, and chromatin structure act as regulatory layers on top of DNA, influencing disease risk far more than previously thought. The conversation dives into how technologies like bisulfite conversion and digital PCR enable precise measurement of these changes, transforming epigenetic signals into actionable data. Dr. Philibert also highlights real-world applications, from detecting behavioral risk factors to enabling early disease biomarkers, emphasizing how epigenetics can’t be “gamed” like traditional reporting methods, making it a powerful tool for precision health interventions. From spilled drink mishaps to science fiction inspiration, the career corner brings humor and humanity. Dr. Philibert reflects on mentorship, passion, and the joy of seeing students turn ideas into impactful innovations, proving that science is as much about people as it is about discovery. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
6 mai

Multiplexing in action – from simplicity to scientific complexity

Multiplexing is reshaping how scientists think about PCR experiments, turning single-target workflows into powerful, multi-dimensional analyses. In this Science Snapshot, we revisit standout moments from past episodes to explore how multiplexing is applied across research areas, from measuring interferon-related genes in lupus to analyzing complex microbial communities and detecting low-level donor-derived DNA. Guests highlight both the opportunities and challenges, including assay design, probe optimization, and troubleshooting strategies. The episode also showcases how multiplexing enables entirely new applications, such as comparing chromosome ratios, assessing molecular integrity, and distinguishing functional mRNA from truncated byproducts. Whether using qPCR or digital PCR, multiplexing allows researchers to extract more information per sample, improve precision through multiple data points, and better reflect biological complexity. As platforms and chemistries continue to evolve, multiplexing is becoming more accessible, helping scientists move faster, conserve material, and focus more on interpreting results rather than optimizing reactions. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
15 avr.

Finding the cheat codes to cell identity

In this episode, Fiona Connolly, former Platform Innovation and Automation Scientist at bit.bio, explains how deterministic programming replaces the traditional “pinball machine” model of stem cell differentiation with precise genetic instructions. By identifying the exact transcription factor combinations, the “cheat codes” that define specific cell fates, Fiona’s team engineers induced pluripotent stem cells (iPSCs) to reliably become neurons, oligodendrocyte-like cells, and other specialized types. She discusses how digital PCR enables accurate transgene copy number validation and multiplexed screening of hundreds of clones, while RNA-seq and qPCR confirm expression fingerprints. The result? Consistent, scalable, high-quality human cell models that accelerate disease research, particularly in areas like neurodegeneration and multiple sclerosis while reducing reliance on animal models. In Career Corner, Fiona shares her journey from curious “why” kid to molecular biologist obsessed with CRISPR, robotics, and automation. Her advice: don’t over-optimize your career path. Stay pluripotent, explore interdisciplinary skills, embrace new tech, and let curiosity guide the way. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
1 avr.

Science happens, and so do mistakes

Science doesn’t just advance through breakthroughs, it advances through mistakes. This Science Snapshot episode embraces a universal truth of research: if you haven’t messed something up in the lab, you probably haven’t been there very long. From chemical reactions gone wrong to experiments derailed by one small oversight, these stories are as relatable as they are unforgettable. In this curated “best of” revisit, Absolute Gene-ius brings together candid lab-fail moments shared by past guests across multiple seasons. You’ll hear stories involving exothermic sodium hydroxide reactions, PCR screening disasters caused by a simple reagent mix-up, forgotten spike-ins during RNA-seq prep, accidental sequencing of human DNA instead of plant viruses, and even being locked inside a cold room on the first week in a new lab. Along the way, the episode highlights the science behind each mistake and the importance of lab safety while reinforcing an important message for scientists at every stage: failure is part of the process, and every mistake becomes a lesson worth remembering. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.

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Inspiring stories and advice from dPCR gene-iuses.

Création

Jordan Ruggieri, Lisa Crawford
Années d’activité

2023 - 2026
Épisodes

44
Classification

Tous publics
Copyright

© 2024 Thermo Fisher Scientific
Site web de l’émission

Absolute Gene-ius