The Single-Cell World Podcast

Catia Moutinho

Welcome to the single-cell world podcast! I am Cátia Moutinho. Here, we disentangle single-cell technology. I will share with you my knowledge, tips, advice, and experiences concerning single-cell research and technology. It doesn’t matter what level of expertise you have in this field. If you are a scientist, this podcast is for you! Listen and learn about this new technology. thesinglecellworld.substack.com

  1. 02/29/2024

    Ep.43: The SMART-seq Family-full length RNA methods

    In this insightful podcast episode, I have the privilege of interviewing Simone Picelli, the Head of the Single-Cell Genomics Platform at the Institute of Ophthalmology Basel. As we talked, we got into the specifics of single-cell RNA sequencing (scRNA-seq) full-length methods from the SMART-seq family. Simone shared valuable insights into various methods within, including SMART-seq, SMART-seq 2, SMART-seq 3, FLASH-seq, FLASH-seq low-amplification, and FLASH-seq with UMI. We explored the unique features and applications of each method, highlighting their contributions to advancing single-cell genomics research.  In addition, we also talked about other common methods and the bigger picture of single-cell sequencing technology, such as the problems it faces and its future. Explore the fascinating world of SMART-seq methods with Simone Picelli - a must-listen episode. Tune in now and learn about the ideal way to study RNA isoforms.  ----more---- USEFUL LINKS: * Simone Picelli Information * LinkedIn * Laboratory Publications * SMART-Seq * SMART-Seq 2 Original Paper * SMART-Seq 2 Method Paper * SMART-seq 3 * SMART-Seq 3 Express * FLASH-seq Protocols: * FLASH-Seq * FLASH-Seq UMI * FLASH-Seq low amplification * Bulk FLASH-Seq Scientist.com: science as a service Support our podcast: pay us a Ko-fi This is a public episode. If you'd like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com/subscribe

    1 hr

About

Welcome to the single-cell world podcast! I am Cátia Moutinho. Here, we disentangle single-cell technology. I will share with you my knowledge, tips, advice, and experiences concerning single-cell research and technology. It doesn’t matter what level of expertise you have in this field. If you are a scientist, this podcast is for you! Listen and learn about this new technology. thesinglecellworld.substack.com