Sage Life & Biomedical Sciences

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Welcome to the official free Podcast site from SAGE for Life & Biomedical Sciences. SAGE is a leading international publisher of journals, books, and electronic media for academic, educational, and professional markets with principal offices in Los Angeles, London, New Delhi, and Singapore.

  1. 12/01/2021

    SLAS Technology Author Talk Tech: Special Collection: AI in Process Automation

    Volume 26 Issue 1, February 2021 Dave Pechter discusses the February SLAS Technology Special Collection AI in Process Automation with Guest Editor Cenk Undey (Amgen), which includes the following papers: Artificial Intelligence Effecting a Paradigm Shift in Drug Development by Masturah Bte Mohd Abdul Rashid Artificial Intelligence (AI) to the Rescue: Deploying Machine Learning to Bridge the Biorelevance Gap in Antioxidant Assaysby Sunday Olakunle Idowu and Amos Akintayo Fatokun Deep Learning and Computer Vision Strategies for Automated Gene Editing with a Single-Cell Electroporation Platform by Cesar Patino, Prithvijit Mukherjee, Vincent Lemaitre, et al.

    13 min

  2. 23/11/2020

    SLAS Discovery Author Insights: High-Throughput Screening for Drugs That Inhibit Papain-Like Protease in SARS-CoV-2

    SLAS Discovery 25.10 December 2020 Rob Howes discusses the paper "High-Throughput Screening for Drugs That Inhibit Papain-Like Protease in SARS-CoV-2" with authors Meredith Gardner (Scripps Research) and Michael Farzan (Scripps Research). This research is part of the December Special Issue, Drug Discovery Targeting COVID-19 with Associate Editor Tim Spicer.

    11 min

  3. 08/10/2020

    SLAS Technology Author Talk Tech: Special Collection: Advances in Technology to Address COVID-19

    Volume 25 Issue 6, December 2020 Dave Pechter discusses the December SLAS Technology Special Collection Advances in Technology to Address COVID-19 with Associate Editor Pak Kin Wong (The Pennsylvania State University), which includes Professor Wong's review Advances in Viral Diagnostic Technologies for Combating COVID-19 and Future Pandemics.

    9 min

  4. 08/10/2020

    SLAS Technology Authors Talk Tech: Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable

    Volume 25 Issue 5, June 2020 Dave Pechter discusses with Varun Bhaskar Kothamachu, Ph.D. (Head of Product, Digi.Bio) & Sabrina Zaini (Head of Operations, Digi.Bio) their featured research article, "Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable."

    14 min

  5. 08/10/2020

    SLAS Technology Authors Talk Tech: Special Issue: Carbohydrate Structure Analysis: Methods and Applications

    SLAS Technology 25.4 August 2020 Dave Pechter discusses with Christian Heiss & Parastoo Azadi regarding the SLAS Technology Special Issue: Carbohydrate Structure Analysis: Methods and Applications.

    13 min

  6. 16/06/2020

    SLAS Discovery Author Insights: Functional Genomics for Target Identification

    Volume 25 Issue 6, July 2020 Podcast Editor Rob Howes discusses the SLAS Discovery 2020 July Special Issue with Guest Editors Davide Gianni and Stuart Farrow. This Special Issue of SLAS Discovery presents the topic "Functional Genomics for Target Identification" and is focused on three strategic pillars that form the foundation of the functional genomics discipline. Articles span a variety of relevant topics, from the development of more translatable models of diseases to the creation of validated screening libraries, technologies and computational pipelines to perturb gene function and enable quantitative interpretation of screening outputs.

    13 min

  7. 03/06/2020

    SLAS Discovery Author Insights: Ion Channels and Relevant Drug Screening Approaches

    Volume 25 Issue 5, June 2020 Podcast Editor Rob Howes discusses the SLAS Discovery 2020 June Special Collection with SLAS Discovery Editorial Board Member Joseph G. McGivern. To read the editorial by Joseph G. McGivern and Mei Ding, click here. This Special Collection of SLAS Discovery presents a special collection reflecting recent advances and continuing challenges in the development and application of assay technologies that are applicable to drug discovery for ion channel targets.

    6 min

  8. 28/04/2020

    SLAS Technology Authors Talk Tech: Traceable Impedance-Based Dispensing and Cloning of Living Single Cells and Impedance-Based Single-Cell Pipetting

    Two articles are discussed: Traceable Impedance-Based Dispensing and Cloning of Living Single Cells: Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using an engineered pipet combined with an impedance-based sensing tip. This technology permits the efficient and traceable isolation of living cells, stem cells, and cancer stem cells that can be individually expanded in culture and transplanted. Impedance-Based Single-Cell Pipetting: Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here, we report the modeling, designing, and testing of a disposable pipet tip integrating a cell sensor based on the Coulter principle. We investigate, test, and discuss the effects of design parameters on the sensor performances with an analytical model. We also describe a system that enables the dispensing of single cells using an instrumented pipet coupled with the sensing tip. Most importantly, this system allows the recording of an impedance trace to be used as proof of single-cell isolation. We assess the performances of the system with beads and cells. Finally, we show that the electrical detection has no effect on cell viability. Link to journal articles: Muller G.; Bonzon D.; Claudinot S.; et al. Traceable Impedance-Based Dispensing and Cloning of Living Single Cells    Bonzon D.; Muller G.; Bureau J.; et al. Impedance-Based Single-Cell Pipetting  Link to issue: http://journals.sagepub.com/toc/jlad/25/3

    13 min
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Welcome to the official free Podcast site from SAGE for Life & Biomedical Sciences. SAGE is a leading international publisher of journals, books, and electronic media for academic, educational, and professional markets with principal offices in Los Angeles, London, New Delhi, and Singapore.

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